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Macherey-Nagel


Macherey-Nagel 

Macherey-Nagel является производителем стационарных фаз для хроматографии и производит колонки для ВЭЖХ и ГХ для широкого спектра применений, включая хиральное разделение. 


Линейка продукции: Nucleodur • Nucleosil • Optima • Chromabond • Chromafil • Nucleoshell • Permabond • Polygosil• Polygoprep

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Обзор продуктов Macherey-Nagel

Доступные фазы NUCLEODUR® 

Phase

Modification

% C

pH range

Particle sizes [µm]

USP

C18 Gravity

octadecyl, high density coating, multi-endcapping

18

1–11

1.8 · 3 · 5

L1

C18 Gravity-SB

octadecyl (monomeric), extensive endcapping

13

1–9

1.8 · 3 · 5

L1

C8 Gravity

octyl, high density coating, multi-endcapping

11

1–11

1.8 · 5

L7

C18 Isis

octadecyl, specially cross-linked modification, endcapped

20

1–10

1.8 · 3 · 5

L1

C18 Pyramid

octadecyl with polar endcapping

14

1–9

1.8 · 3 · 5

L1

PolarTec

octadecyl with embedded polar group

17

1–9

1.8 · 3 · 5

L1 L60

Phenyl-Hexyl

phenylhexyl, multi-endcapping

10

1–10

1.8 · 3 · 5

L11

π2

biphenylpropyl, multi-endcapping

17

1.5–10

5

L11

PFP

pentafluorophenylpropyl, 
multi-endcapping

8

1–9

1.8 · 3 · 5

L43

Sphinx RP

bifunctional, balanced ratio of propylphenyl and octadecyl, endcapping

15

1–10

1.8 · 3 · 5

L1 L11

C18 HTec

octadecyl, high density coating, high capacity, multi-endcapping

18

1–11

1.8 · 3 · 5 
7 · 10

L1

C18 ec

octadecyl, medium density, endcapping

17.5
4

1–9

3 · 5 
and larger

L1

C8 ec

octyl, medium density, endcapping

10.5

1–9

3 · 5

L7

C4 ec

butyl, medium density, endcapping

2.5

1–9

5

L26

HILIC

ammonium – sulfonic acid

7

2–8.5

1.8 · 3 · 5

 

CN/CN-RP

cyano (nitrile) for NP and RP separations

7

1–8

3 · 5

L10

NH2/NH2-RP

aminopropyl for NP and RP separations

2.5

2–8

3 · 5

L8

SiOH

unmodified NUCLEODUR®

2–8

3 · 5 
and larger

L3

 

 

Доступные фазы NUCLEOSHELL® 

Phase

Modification

% C

Particle size [µm]

pH range

USP

RP 18

Octadecyl, multi-endcapping

7.8

2.7

1–11

L1

6.1

5

RP 18plus

Octadecyl (monomeric), multi-endcapping

5.7

2.7

2–9

L1

4.4

5

Phenyl-Hexyl

Phenyl-Hexyl, multi-endcapping

4.5

2,7

1–10

L11

PFP

Pentafluorophenyl, multi-endcapping

~ 3

2,7

1–9

L43

HILIC

Ammonium – sulfonic acid

1.3

2,7

2–8.5

 

Demands on HPLC separations are constantly increasing with respect to separation efficiency and detection limits as well as concerning the time requirements for each analysis. Core-shell technology sets new standards for increasing efficiency in research and quality control.

 

Benefits of core-shell technology

Core-shell particles vs. 
totally porous silica gel

Short diffusion paths

 
 

Rapid mass transfer (C term of the Van Deemter equation)

 

High flow velocity without peak broadening for fast LC

Narrow particle size distribution (d90/d10 ~ 1.1)

 

Stable packing

High heat transfer

 

Minimized influence of frictional heat

 

Column efficiency of NUCLEOSHELL® ~ 250000/m (HETP ~ 4 µm)

 

Resolution Rs as function of particle size

Columns:

50 x 4 mm
NUCLEOSHELL® RP 18, 2.7 µm
NUCLEODUR® C18 Gravity, 3 µm
NUCLEODUR® C18 Gravity, 1.8 µm

 

Eluent:
Flow rate:
Temperature:
Detection:

acetonitrile – water (60:40, v/v)
1 mL/min
25 °C
UV, 254 nm

Peaks:
1. Naphthalene
2. Ethylbenzene

Better resolution at
lower back pressure and 
shorter retention time

 

 

Доступные фазы NUCLEOSIL® 

Phase

Modification

Formula

RP phases

NUCLEOSIL® C18

medium density octadecyl, endcapped

-(CH2)17-CH3

NUCLEOSIL® C18 AB

crosslinked octadecyl, endcapped, high steric selectivity

-(CH2)17-CH3

NUCLEOSIL® C18 HD

high density octadecyl, endcapped

-(CH2)17-CH3

NUCLEOSIL® C18 Nautilus

hydrophilic octadecyl, embedded polar group, endcapped

-(CH2)17-CH3

NUCLEOSIL® Protect I

special RP phase with protective polar group, endcapped

 

NUCLEOSIL® C8 ec

medium density octyl, endcapped

-(CH2)7-CH3

NUCLEOSIL® C8

standard octyl, not endcapped

-(CH2)7-CH3

NUCLEOSIL® C8 HD

high density octyl, endcapped

-(CH2)7-CH3

NUCLEOSIL® C4

medium density butyl, endcapped

-(CH2)3-CH3

NUCLEOSIL® C2

dimethyl

-(CH3)2

NUCLEOSIL® C6H5

standard phenyl, not endcapped

-(CH2)3-C6H5

Polar phases

NUCLEOSIL® CN

cyanopropyl (nitrile)

-(CH2)3-CN

NUCLEOSIL® OH

diol (dihydroxypropyl)

-(CH2)3-O-CH2-CH(OH)-CH2OH

NUCLEOSIL® NH2

aminopropyl

-(CH2)3-NH2

NUCLEOSIL® N(CH3)2

dimethylamino

-(CH2)3-N(CH3)2

NUCLEOSIL® SA

benzenesulfonic acid, strongly acidic cation exchanger (SCX)

-(CH2)3-C6H4-SO3Na

NUCLEOSIL® SB

quaternary ammonium groups, strongly basic anion exchanger (SAX)

-(CH2)3-C6H4-CH2-N+(CH3)3Cl

 

 

 

NUCLEOSIL® SiOH

unmodified

(SiO2)n

 

Технические характеристики

 

Spherical silica, pH stability 2–8
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm

 

Physical properties of unmodified NUCLEOSIL® materials

 

Phase

Pore size

Pore volume

Surface (BET)

Density

Pressure stability*

 

NUCLEOSIL 50

50 Å

0.8 mL/g

420 m2/g

0.45 g/mL

500 bar

 

NUCLEOSIL 100

100 Å

1 mL/g

350 m2/g

0.36 g/mL

500 bar

 

NUCLEOSIL 120

120 Å

0.65 mL/g

200 m2/g

0.55 g/mL

500 bar

 

NUCLEOSIL 300

300 Å

0.8 mL/g

100 m2/g

0.45 g/mL

400 bar

 

NUCLEOSIL 500

500 Å

0.8 mL/g

35 m2/g

0.45 g/mL

400 bar

 

NUCLEOSIL 1000

1000 Å

0.8 mL/g

25 m2/g

0.45 g/mL

300 bar

 

NUCLEOSIL 4000

4000 Å

0.7 mL/g

10 m2/g

0.48 g/mL

300 bar

 

* Maximum packing pressure; for maximum working pressure of packed columns please refer to the instruction leaflets.